Effects of a 50 Hz sinusoidal magnetic field on cell adhesion molecule expression in two human osteosarcoma cell lines (MG‐63 and Saos‐2)
- 16 June 2003
- journal article
- research article
- Published by Wiley in Bioelectromagnetics
- Vol. 24 (5), 327-338
- https://doi.org/10.1002/bem.10113
Abstract
The possibility that a sinusoidal 50 Hz magnetic field with a magnetic flux density of 0.5 mT can induce variations in the expression of cell adhesion molecules (CAMs) in two human osteosarcoma cell lines (MG‐63 and Saos‐2) was investigated. In particular, the expression of two important integrins, VLA‐2, the receptor for collagen, and VLA‐5, the receptor for fibronectin, as well as CD44, were examined in both cell lines after these had been exposed for 7 and 14 days to a 50 Hz, 0.5 mT field. Cell surface morphology (scanning electron microscopy), cell growth characteristics (growth curves and cell cycle phase distribution), and cell death (necrosis and apoptosis) were also examined. The results demonstrate that no variations in surface morphology and cell death occurred between control and exposed cells in both MG‐63 and Saos‐2 cells, while significant changes were noted in cell growth and fibronectin and CD44 expression in MG‐63 cells. The results are discussed in view of the important role that CAMs play in controlling various cancer cell functions, particularly proliferation and metastasis. Bioelectromagnetics 24:327‐338, 2003.Keywords
This publication has 42 references indexed in Scilit:
- Exposure of cells to static magnetic field accelerates loss of integrity of plasma membrane during apoptosisCytometry, 2002
- Role of the αvβ6 Integrin in Human Oral Squamous Cell Carcinoma Growth in Vivo and in VitroBiochemical and Biophysical Research Communications, 2001
- Occupational exposure to magnetic fields relative to mortality from brain tumours: updated and revised findings from a study of United Kingdom electricity generation and transmission workers, 1973–97Occupational and Environmental Medicine, 2001
- Extremely low frequency electromagnetic fields (EMF) and brain cancer in adults and children: Review and commentNeuro-Oncology, 1999
- Investigation of the effects of 50 Hz magnetic fields on purified human hematopoietic progenitorsLife Sciences, 1997
- Apoptosis — the cell's silent exitLife Sciences, 1996
- Hepatocyte death following transforming growth factor-β1 additionMicroscopy Research and Technique, 1996
- Residential proximity to electricity transmission and distribution equipment and risk of childhood leukemia, childhood lymphoma, and childhood nervous system tumors: systematic review, evaluation, and meta-analysisCancer Causes & Control, 1994
- Systemic reaction of Escherichia coli cells to weak electromagnetic fields of extremely low frequencyBioelectrochemistry and Bioenergetics, 1994
- Integrins: Versatility, modulation, and signaling in cell adhesionCell, 1992