ISOLATION OF A GROUP OF GLYCOLIPID TOXINS FROM SEEDHEADS OF ANNUAL RYEGRASS Lolium rigidum GAUD.) INFECTED BY Corynebacterium rathayi
- 1 August 1981
- journal article
- research article
- Published by Wiley in Immunology & Cell Biology
- Vol. 59 (4), 455-467
- https://doi.org/10.1038/icb.1981.39
Abstract
A group of highly toxic compounds was isolated from galled seedheads of annual ryegrass (Lolium rigidum Gaud.) containing Corynebacterium rathayi. Purified extracts were resolved by reverse-phase high-performance liquid chromatography into eight main fractions which have been partially characterised and shown to be toxic to nursling rats. A mixture of the toxins also produced clinical signs and brain lesions in Iambs consistent with annual ryegrass toxicity. The name 'corynetoxin' is tentatively proposed for the series, individual members being designated according to their order of elution from the high performance liquid chromatography column as corynetoxins 1 to 8. The two main fractions are corynetoxins 3 and 4 of which the former has been crystallised. They appear to be of glycolipid character, 3-hydroxyheptadecanoic acid and a C6 amino sugar being identified among the hydrolysis products of corynetoxin 3, and heptadec-2-enoic acid and a C6 amino sugar from corynetoxin 4.Keywords
This publication has 4 references indexed in Scilit:
- Production of a neurotoxin in a cell culture of endosperm from Lolium multiflorum colonised by Corynebacterium rathayiPhysiological Plant Pathology, 1980
- BRAIN DAMAGE BY EXTRACTS OF PARASITISED ANNUAL RYEGRASS (LOLIUM RIGIDUM) IN NURSLING RATSImmunology & Cell Biology, 1977
- Optimum conditions for bonding of plant phenols to insoluble polyvinylpyrrolidonePhytochemistry, 1968
- Chemical constitution of a glycolipid from C. diphtheriae P.W.BBiochemical and Biophysical Research Communications, 1963