A new system for ATP‐metric immunoanalysis
- 17 October 1983
- journal article
- Published by Wiley in FEBS Letters
- Vol. 162 (2), 266-269
- https://doi.org/10.1016/0014-5793(83)80769-8
Abstract
Treatment of amino‐group‐containing antigens with adenosine‐5′‐trimetaphosphate results in their chemical modification by ‐pppA residues. An immunoanalytical system is proposed based upon competition of these ATP‐labelled antigens with those of the sample for immobilized antibodies. Mild acidic treatment of complexes of ATP‐labelled antigens with immobilized antibodies results in quantitative liberation of intact ATP. The latter may be determined by the ultrosensitive bioluminescent techniques based upon emission of light with firefly luciferase. The validity of the system has been studied with two clinically important antigens, thyroxine and myoglobin.This publication has 5 references indexed in Scilit:
- A rapid method for myoglobin radioimmunoanalysis as a diagnostic tool in myocardial infarctionClinica Chimica Acta; International Journal of Clinical Chemistry, 1982
- Alternatives to radioimmunoassay: labels and methods.Clinical Chemistry, 1981
- General method for the synthesis of ATP gamma‐derivativesFEBS Letters, 1976
- Specific protein-binding reactions monitored with ligand-ATP conjugates and firefly luciferaseAnalytical Biochemistry, 1976
- The Measurement of Pyridine Nucleotides by Enzymatic CyclingJournal of Biological Chemistry, 1961