Sequence and expression of the rat prodynorphin gene.

Abstract

The isolation of a .lambda. genomic clone that contains the nucleotide sequence coding for the main exon of the rat prodynorphin (proenkephalin B) gene is reported. This exon codes for the majority of the translated region of prodynorphin mRNA including the opioid peptides .alpha.-neoendorphin, dynorphin A and dynorphin B. The entire 3'' untranslated region is also contained on the .lambda. clone. Nucleotide sequence comparison with the main exon of the human prodynorphin gene reveals both structural and sequence homology. RNA blot analysis reveals that prodynorphin transcripts can be seen in numerous regions of the rat brain and in the adrenal gland, spinal cord, testis and anterior pituitary.