DNA sequence of an Immediate-early gene (IE mRNA-5) of herpes simplex virus type I
Open Access
- 11 February 1982
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 10 (3), 979-991
- https://doi.org/10.1093/nar/10.3.979
Abstract
We describe a 2560 base pair herpes simplex virus type 1 (HSV-1) DNA sequence containing the entire immediate-early mRNA-5 (IEmRNA-5) gene. The 3′ and 5′ termini of IEmRNA-5 were mapped within this DNA sequence by single-strand specific endonuclease protection experiments. The IEmRNA-5 gene contains DNA sequences from both the unique (Us) and reiterated (TRs/IRs) regions of the HSV-1 DNA short component and is interrupted by a single intron mapping in TRs/IRs. A search of the transcribed DNA sequence revealed no initiator codon within TRs/IRs. The first ATG was located 6 bases into Us sequences and this reading frame was open for a further 87 codons. A second longer open reading frame (316 codons) was also observed in the 3′ transcribed region. The oligonucleotide sequences adjacent to the IEmRNA-5 termini are discussed in relation to those of the HSV-1 thymidine kinase gene and other genes transcribed by RNA polymerase II.Keywords
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