Rapid Detection of Polymyxin-Resistant Enterobacteriaceae from Blood Cultures
- 1 September 2016
- journal article
- research article
- Published by American Society for Microbiology in Journal of Clinical Microbiology
- Vol. 54 (9), 2273-2277
- https://doi.org/10.1128/jcm.00918-16
Abstract
Enterobacterial strains resistant to polymyxins are being increasingly reported worldwide. The conventional methods for detection of colistin-resistant isolates such as broth microdilution remain time-consuming (24 to 48 h), and methods such as disc diffusion and Etest are not reliable. Recently, the rapid polymyxin NP test was developed for rapid identification of polymyxin-resistant Enterobacteriaceae . This test is based on the detection of glucose metabolism related to bacterial growth in the presence of a defined concentration of colistin (or polymyxin B). The formation of acid metabolites is evidenced by a color change of a pH indicator (red phenol) in less than 2 h. In this study, the polymyxin NP test was evaluated for detection of colistin-resistant Enterobacteriaceae directly from blood cultures. The test was performed with 73 blood culture sets (either spiked or clinical blood cultures) with various enterobacterial species. The test exhibited excellent discrimination between polymyxin-resistant and polymyxin-susceptible enterobacterial isolates, and results are obtained from blood cultures within 4 h. It is easy to perform and requires neither subculture nor a centrifugation step. This test is rapid, specific, and sensitive and allows early identification of polymyxin-resistant Enterobacteriaceae directly from blood cultures.Keywords
Funding Information
- University of Fribourg, Switzerland
This publication has 21 references indexed in Scilit:
- Susceptibility Testing of the Polymyxins: Where Are We Now?Pharmacotherapy: The Journal of Human Pharmacology and Drug Therapy, 2014
- The mgrB gene as a key target for acquired resistance to colistin in Klebsiella pneumoniaeJournal of Antimicrobial Chemotherapy, 2014
- The difficult-to-control spread of carbapenemase producers among Enterobacteriaceae worldwideClinical Microbiology & Infection, 2014
- Resistance to Colistin Associated with a Single Amino Acid Change in Protein PmrB among Klebsiella pneumoniae Isolates of Worldwide OriginAntimicrobial Agents and Chemotherapy, 2014
- Rapid detection of carbapenemase-producing Enterobacteriaceae from blood culturesClinical Microbiology & Infection, 2014
- Comparison of disc diffusion, Etest and agar dilution for susceptibility testing of colistin against EnterobacteriaceaeLetters in Applied Microbiology, 2011
- Initiation of Inappropriate Antimicrobial Therapy Results in a Fivefold Reduction of Survival in Human Septic ShockChest, 2009
- Mortality and morbidity attributable to inadequate empirical antimicrobial therapy in patients admitted to the ICU with sepsis: a matched cohort studyJournal of Antimicrobial Chemotherapy, 2007
- Comparative Evaluation of the VITEK 2, Disk Diffusion, Etest, Broth Microdilution, and Agar Dilution Susceptibility Testing Methods for Colistin in Clinical Isolates, Including Heteroresistant Enterobacter cloacae and Acinetobacter baumannii StrainsAntimicrobial Agents and Chemotherapy, 2007
- Comparison of Etest, Vitek and agar dilution for susceptibility testing of colistinClinical Microbiology & Infection, 2007