Quantitative Lipoprotein Studies in Normal and Abnormal Subjects Using Combined Electrophoretic and Chemical Technics

Abstract

Summary 1) A method for paper electro-phoretic separation and quantitative determination of serum alpha and beta lipoprotein neutral fat, cholesterol and phospholipid, respectively, is described. Studies in 9 normal and 5 abnormal subjects are included. 2) The sums of total lipid, cholesterol and phospholipid recovered after electrophoretic separation of alpha and beta lipoprotein are compared with those values determined macro-chemically in untreated sera. Separation of abnormal from normal individuals appears to have been achieved in this small series in terms of distribution of phospholipid, and possibly of cholesterol, in the alpha and beta lipoprotein complexes.