Autoradiographic Imaging of Phosphoinositide Turnover in the Brain
- 17 August 1990
- journal article
- research article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 249 (4970), 802-804
- https://doi.org/10.1126/science.1975122
Abstract
With [3H]cytidine as a precursor, phosphoinositide turnover can be localized in brain slices by selective autoradiography of the product [3H]cytidine diphosphate diacylglycerol, which is membrane-bound. In the cerebellum, glutamatergic stimulation elicits an increase of phosphoinositide turnover only in Purkinje cells and the molecular layer. In the hippocampus, both glutamatergic and muscarinic cholinergic stimulation increase phosphoinositide turnover, but with distinct localizations. Cholinergic stimulation affects CA1, CA3, CA4, and subiculum, whereas glutamatergic effects are restricted to the subiculum and CA3. Imaging phosphoinositide turnover in brain slices, which are amenable to electrophysiologic studies, will permit a dynamic localized analysis of regulation of this second messenger in response to synaptic stimulation of specific neuronal pathways.This publication has 20 references indexed in Scilit:
- Messenger molecules in the cerebellumTrends in Neurosciences, 1990
- Inhibition of Excitatory Amino Acid‐Stimulated Phosphoinositide Hydrolysis in the Neonatal Rat Hippocampus by 2‐Amino‐3‐PhosphonopropionateJournal of Neurochemistry, 1989
- Inositol phosphates and cell signallingNature, 1989
- The Excitatory Amino Acid Receptors: Their Classes, Pharmacology, and Distinct Properties in the Function of the Central Nervous SystemAnnual Review of Pharmacology and Toxicology, 1989
- Long-Term Synaptic PotentiationScience, 1988
- The current excitement in long term potentiationNeuron, 1988
- NMDA receptors - their role in long-term potentiationTrends in Neurosciences, 1987
- The mismatch problem in receptor mapping studiesTrends in Neurosciences, 1985
- Light autoradiographic localisation of cholinergic muscarinic receptors in rat brain by specific binding of a potent antagonistNature, 1975
- QUANTITATIVE HISTOCHEMISTRY OF ACETYL‐CHOLINESTERASE IN RAT HIPPOGAMPAL REGION CORRELATED TO HISTOCHEMICAL STAININGJournal of Neurochemistry, 1970