Purification and Characterization of Alanine Carrier Isolated from H-Proteins of Bacillus subtilis
- 1 February 1978
- journal article
- research article
- Published by Wiley in European Journal of Biochemistry
- Vol. 83 (1), 307-311
- https://doi.org/10.1111/j.1432-1033.1978.tb12095.x
Abstract
Alanine transport carrier was isolated and purified from H‐proteins of Bacillus subtilis. The purified carrier preparation was homogeneous in migration on polyacrylamide gels containing urea or sodium dodecyl sulfate. Electrophoresis on polyacrylamide gels containing dodecyl sulfate showed a single band of molecular weight of about 7500. 1 mol alanine was bound/mol carrier protein with a dissociation constant of 0.2 μM. The binding was inhibited by p‐chloromercuribenzoate and the inhibition was reversed by dithiothreitol.This publication has 10 references indexed in Scilit:
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