Effects of Phagocytosis on Antibiotic and Nucleoside Uptake by Human Polymorphonuclear Leukocytes
- 1 March 1984
- journal article
- research article
- Published by Oxford University Press (OUP) in The Journal of Infectious Diseases
- Vol. 149 (3), 397-403
- https://doi.org/10.1093/infdis/149.3.397
Abstract
The ability of antibiotics to enter phagocytes during infection with facultative intracellular organisms was investigated using an in vitro model. Human polymorphonuclear leukocytes (PMNLs) were incubated with ingestible particles or phorbol myristate acetate (PMA), after which radiolabeled antibiotics were added to the cell suspension. Antibiotic uptake, determined by a velocity-gradient centrifugation technique, was expressed as the cellular:extracellular (C/E) antibiotic concentration ratio. Phagocytosis or PMA exposure enhanced PMNL clindamycin uptake (for example, C/E ratio of 12 for control vs 30 after zymosan). Entry of penicillin was unaffected and erythromycin uptake was slightly decreased after phagocytosis. Because clindamycin uptake by phagocytes is mediated by the nucleoside transport system, adenosine uptake after phagocytosis was studied. Adenosine uptake was stimulated by phagocytosis, and this increase was inhibited by clindamycin. Thus, clindamycin uptake, mediated by the nucleoside transport system, was augmented by phagocytosis. This marked stimulation of a membrane transport system by phagocytosis has not been previously described.Keywords
This publication has 10 references indexed in Scilit:
- Energy Metabolism of Human Neutrophils during PhagocytosisJCI Insight, 1982
- Antibiotic entry into human polymorphonuclear leukocytesAntimicrobial Agents and Chemotherapy, 1982
- Cellular Uptake and Intracellular Activity of Antibiotics Against Haemophilus influenzae Type bThe Journal of Infectious Diseases, 1982
- Membrane transport of clindamycin in alveolar macrophagesAntimicrobial Agents and Chemotherapy, 1982
- PHAGOCYTOSIS-INDUCED MODULATION OF HUMAN NEUTROPHIL CHEMOTAXIS RECEPTORS1981
- MEMBRANE FLUIDITY CHANGES ACCOMPANYING PHAGOCYTOSIS IN NORMAL AND IN CHRONIC GRANULOMATOUS-DISEASE POLYMORPHONUCLEAR LEUKOCYTES1981
- ANTIBIOTIC UPTAKE BY ALVEOLAR MACROPHAGES1980
- Effect of phagocytosis by human polymorphonuclear leukocytes and rabbit alveolar macrophages on 2-deoxyglucose transportJournal of Cellular Physiology, 1979
- EFFECT OF ANTIBIOTICS ON THE INTRACELLULAR SURVIVAL OF STAPHYLOCOCCUS-AUREUS INVITRO1979
- Membrane transport by guinea pig peritoneal exudate leukocytes: Effect of phagocytosis on hexose and amino acid transportJournal of Cellular Physiology, 1977