Adhesions in a murine flexor tendon graft model: Autograft versus allograft reconstruction
Open Access
- 9 January 2008
- journal article
- research article
- Published by Wiley in Journal of Orthopaedic Research
- Vol. 26 (6), 824-833
- https://doi.org/10.1002/jor.20531
Abstract
Reconstruction of flexor tendons often results in adhesions that compromise joint flexion. Little is known about the factors involved in the formation of flexor tendon graft adhesions. In this study, we developed and characterized a novel mouse model of flexor digitorum longus (FDL) tendon reconstruction with live autografts or reconstituted freeze‐dried allografts. Grafted tendons were evaluated at multiple time points up to 84 days post‐reconstruction. To assess the flexion range of the metatarsophalangeal joint, we developed a quantitative outcome measure proportional to the resistance to tendon gliding due to adhesions, which we termed the Gliding Coefficient. At 14 days post‐grafting, the Gliding Coefficient was 29‐ and 26‐fold greater than normal FDL tendon for both autografts and allografts, respectively (p < 0.001), and subsequently doubled for 28‐day autografts. Interestingly, there were no significant differences in maximum tensile force or stiffness between live autograft and freeze‐dried allograft repairs over time. Histologically, autograft healing was characterized by extensive remodeling and exuberant scarring around both the ends and the body of the graft, whereas allograft scarring was abundant only near the graft–host junctions. Gene expression of GDF‐5 and VEGF were significantly increased in 28‐day autografts compared to allografts and to normal tendons. These results suggest that the biomechanical advantages for tendon reconstruction using live autografts over devitalized allografts are minimal. This mouse model can be useful in elucidating the molecular mechanisms in tendon repair and can aid in preliminary screening of molecular treatments of flexor tendon adhesions. © 2008 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 26:824–833, 2008Keywords
This publication has 43 references indexed in Scilit:
- Patterns of mRNA Expression for Matrix Molecules and Growth Factors in Flexor Tendon Injury: Differences in the Regulation Between Tendon and Tendon SheathThe Journal of Hand Surgery, 2006
- Neutralisation of TGFβ or binding of VLA-4 to fibronectin prevents rat tendon adhesion following transectionCytokine, 2005
- Quantitative variation in vascular endothelial growth factor mRNA expression during early flexor tendon healing: an investigation in a canine modelJournal of Orthopaedic Research, 2001
- Modulation of the formation of adhesions during the healing of injured tendonsThe Journal of Bone and Joint Surgery, 2000
- Effects of increased in vivo excursion on digital range of motion and tendon strength following flexor tendon repairJournal of Orthopaedic Research, 1999
- Molecular studies in flexor tendon wound healing: The role of basic fibroblast growth factor gene expressionThe Journal of Hand Surgery, 1998
- The Marshall R. Urist Young Investigator AwardPublished by Wolters Kluwer Health ,1997
- Tendon excursions after flexor tendon repair in zone II: Results with a new controlled-motion programThe Journal of Hand Surgery, 1993
- Bridge Flexor Tendon GraftsPublished by Wolters Kluwer Health ,1989
- The Use of Freeze-dried Allografts in the Management of Global Rotator Cuff TearsPublished by Wolters Kluwer Health ,1988