Inhibition of nerve growth factor‐induced neurite outgrowth of PC12 cells by a protein kinase inhibitor which does not permeate the cell membrane
- 1 November 1991
- journal article
- Published by Wiley in FEBS Letters
- Vol. 293 (1-2), 119-123
- https://doi.org/10.1016/0014-5793(91)81165-5
Abstract
K-252a, a protein kinase inhibitor of microbial origin, has proven to be a specific inhibitor of nerve growth factor. In this study, the effects of K-252b, the 9-carboxylic acid derivative of K-252a, on nerve growth factor-induced neurite outgrowth in PC12 cells was examined. K-252b is hydrophilic and does not permeate the cell membrane of PC12 cells, whereas K-252a clearly does. K-252b is, however, as potent as K-252a itself in inhibiting the nerve growth factor-induced neurite outgrowth. These results can be interpreted to suggest that effects of K-252b may be through surface-bound/anchored K-252b-sensitive molecules on PC12 cellsKeywords
This publication has 32 references indexed in Scilit:
- K‐252b Is a Selective and Nontoxic Inhibitor of Nerve Growth Factor Action on Cultured Brain NeuronsJournal of Neurochemistry, 1991
- Basic fibroblast growth factor is a substrate for phosphorylation by human neutrophil ecto-protein kinase activityBiochemical and Biophysical Research Communications, 1991
- K252a is a potent and selective inhibitor of phosphorylase kinaseBiochemical and Biophysical Research Communications, 1990
- K-252a inhibits the increase in c-fos transcription and the increase in intracellular calcium produced by nerve growth factor in PC12 cellsJournal of Neuroscience Research, 1989
- Inhibition by K-252a, a new inhibitor of protein kinase, of nerve growth factor-induced neurite outgrowth of chick embryo dorsal root ganglion cellsNeuroscience Letters, 1988
- Functional synapse formation between cultured neurons of rat cerebral cortex. Block by a protein kinase inhibitor which does not permeate the cell membrane.Proceedings of the Japan Academy, Series B, 1988
- K-252 compounds, novel and potent inhibitors of protein kinase C and cyclic nucleotide-dependent protein kinasesBiochemical and Biophysical Research Communications, 1986
- A Nerve Growth Factor-Sensitive S6 Kinase in Cell-Free Extracts from PC 12 CellsJournal of Neurochemistry, 1986
- Phosphorylation of external cell-surface proteins by an endogenous ecto-protein kinase of goat epididymal intact spermatozoaBiochimica et Biophysica Acta (BBA) - Molecular Cell Research, 1986
- Nerve growth factor mediates phosphorylation of specific proteinsCell, 1980