Differential contribution of Mr 120 kDa rasGTPase‐activating protein and neurofibromatosis type 1 gene product during the transition from growth phase to arrested state in human fibroblasts accompanied by a unique rasGTPase‐activating active
- 26 July 1993
- journal article
- Published by Wiley in FEBS Letters
- Vol. 327 (2), 177-182
- https://doi.org/10.1016/0014-5793(93)80165-q
Abstract
Using octyl glucoside-solubilized cell extracts from human fibroblasts during growth phase to G0/G1 arrest state, we found that while the number of M r, 120 kDa rasGTPase-activating protein (p120GAP) molecules per cell decreases to half its original levels, the amount of neurofibromatosis type 1 gene product (NF1, neurofibromin) remains constant during the transition. The contribution of pl20GAP to the total rasGTPase-activating (rasGA) activity in growing cells was found to be larger than that observed in arrested cells (84% vs 53%). On the other hand, NF1 contributes less than 15% of the total rasGA activity in either extract. These results indicate that the qualitative changes occur in the contributors to rasGA activity during transition. They also suggest that a unique rasGA activity exists in the arrested cells, which was obtained separatedly from both pl20GAP and NF1 by heparin-Sepharose column chromatography.Keywords
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