Abstract
The biochemical reactions of 18 anaerogenic and 45 aerogenic isolates of E. agglomerans are described and used for subdivision in the biogroups suggested by Ewing and Fife. The presence of special cultural characteristics (symplasmata and biconvex bodies) was also recorded and was unrelated to formation of gas from glucose. Previous authors reported such structures in anaerogenic cultures only. The amount of gas produced varied to a great extent between strains and was dependent on incubation temperature. The results do not support the division of E. agglomerans into 1 anaerogenic and 1 aerogenic major subgroup. The value of the gelatin liquefaction test for characterization of the species is emphasized.

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