Bvr-1, a restriction locus of a type C RNA virus in the feline cellular genome: pleiotropic restriction of endogenous BALB virus in cat X mouse somatic cell hybrids.
Open Access
- 1 January 1978
- journal article
- research article
- Published by Rockefeller University Press in The Journal of Experimental Medicine
- Vol. 147 (1), 219-232
- https://doi.org/10.1084/jem.147.1.219
Abstract
Bvr-1 is a dominant X-linked feline gene which restricts the replication of B-tropic murineleukemia virus (B-MuLV) in somatic cell hybrids between murine BALB/c-RAG cells and FL-74 feline cells. Since the hybrids were originally derived by the hypoxanthine aminopterin thymidine selection scheme, counter selection experiments on 6-thioguanine result in preferential survival of hybrid cells which have spontaneously lost the feline X-chromosome on which is located the structural gene for hypoxanthine guanine phosphoribosyl transferase (IMP: pyrophosphate phosphoribosyl transferase, E.C. 2.4.2.8) and Bvr-1. Back selected Bvr-1- cells express high parental levels of B-MuLV. Bvr-1 effectively restricts the IdU-mediated induction of the endogenous xenotropic BALB virus (BALB: virus 2) but not the endogenous N-tropic virus (BALB: virus 1). Pleiotropic restriction of B-MuLV and X-MuLV, but not N-MuLV suggests that the viral targets of Bvr-1 (either viral components or functions in viral assembly) of the B-tropic and X-tropic endogenous BALB viruses are similar to each other but distinct from the target in the N-tropic virus. Very low levels of B-MuLV are detected in restricted cells, but this residual virus is not infectious in either NIH-3T3 or BALB-3T3 mouse cells which are genotypically Fv-1N/Fv-1N and Fv-1B/Fv-1B, respectively. Passage of residual virus through host cells without Fv-1 related restriction (SC-1) results in production of infectious B-MuLV indistinguishable from that produced by RAG parent cells.This publication has 32 references indexed in Scilit:
- Expression of Feline Leukaemia Virus Antigens on Cat Lymphoma Cells: Kinetics of BiosynthesisJournal of General Virology, 1977
- Expression of Virus Structural Proteins on Murine Cell Surfaces in Association with the Production of Murine Leukaemia VirusJournal of General Virology, 1976
- Effect of Fv-1 gene product on synthesis of N-tropic and B-tropic murine leukemia viral RNACell, 1976
- Isolation from BALB/c mouse cells of a structural polypeptide of a third endogenous type C virusCell, 1974
- Xenotropic Viruses: Murine Leukemia Viruses Associated with NIH Swiss, NZB, and Other Mouse StrainsScience, 1973
- Murine C-type RNA Virus from Spontaneous Neoplasms: in vitro Host Range and Oncogenic PotentialScience, 1973
- STUDIES OF GENETIC TRANSMISSION OF MURINE LEUKEMIA VIRUS BY AKR MICEThe Journal of Experimental Medicine, 1972
- Prevalence of the group‐specific (gs) antigen and infectious virus expressions of the murine C‐type RNA viruses during the life span of BALB/cCr miceInternational Journal of Cancer, 1972
- CONTROLLED PRODUCTION OF PROLIFERATING SOMATIC CELL HYBRIDSThe Journal of cell biology, 1970
- Development of 3T3‐like lines from Balb/c mouse embryo cultures: Transformation susceptibility to SV40Journal of Cellular Physiology, 1968