ISOLATION AND PROPERTIES OF LIVER CELL NUCLEOLI
Open Access
- 25 March 1956
- journal article
- research article
- Published by Rockefeller University Press in The Journal of cell biology
- Vol. 2 (2), 127-145
- https://doi.org/10.1083/jcb.2.2.127
Abstract
Cytological changes occurring in young oocytes of the mouse, following whole-body x-irradiation, have been examined by light and electron microscopy. Two minutes after a dose of 200 r, a drop occurs in the number of mitochondria per oocyte. The normal number of mitochondria is restored in the next few minutes. Five to 6 hours after a dose of 7 r, and 30 to 45 minutes following a dose of 200 r, all the oocytes are markedly contracted. In the next 2 hours (at a dose of 7 r), some cells shrink further and become pycnotic, while others expand and show signs of karyolysis. Of the expanded cells, some (about 50 per cent of total young oocytes) become morphologically normal. In both pycnotic and karyolytic nuclei, the nucleolus was contracted but without loss of its fibrillar structure. The contracted nucleolus appeared similar to those seen in some nearly mature normal oocytes (these oocytes have a high natural death rate). No other cell type in the ovary was affected by doses of x-rays up to 200 r. Observations on the nucleus of the normal oocyte are included. In the dictyate stage of meiotic prophase the chromosomes were dispersed into bundles of 100 A microfibrils. The main component of the nucleolus was found to be tightly coiled fibrils of 60 to 100 A, which appear to have a close relationship with the microfibrils of the chromosomes.Keywords
This publication has 19 references indexed in Scilit:
- A METHOD FOR ISOLATING INTACT MITOCHONDRIA AND NUCLEI FROM THE SAME HOMOGENATE, AND THE INFLUENCE OF MITOCHONDRIAL DESTRUCTION ON THE PROPERTIES OF CELL NUCLEIThe Journal of cell biology, 1955
- Pores in the mammalian nuclear membraneBiochimica et Biophysica Acta, 1954
- Presence of Zinc in Nucleoli and its Possible Role in MitosisNature, 1954
- Observations sur le rôle biochimique du nucléoleBiochimica et Biophysica Acta, 1954
- The Isolation and Biochemical Characterization of the Mitotic Apparatus of Dividing CellsProceedings of the National Academy of Sciences, 1952
- CYTOCHEMICAL STUDIES .6. THE SYNTHESIS OF DIPHOSPHOPYRIDINE NUCLEOTIDE BY LIVER CELL NUCLEI1952
- Structure of the nucleolus as revealed by the electron microscope; a preliminary report.1951
- Simplification of the Manufacture of Schiff Reagent for use in Histochemical ProceduresStain Technology, 1951
- FURTHER STUDIES ON THE KINETICS AND DETERMINATION OF ALDOLASEJournal of Biological Chemistry, 1950
- The Nature of the Specificity of Methyl Green for ChromatinProceedings of the National Academy of Sciences, 1949