THE RELATIONSHIP BETWEEN STEM CELL SEEDING EFFICIENCY AND POSITION IN CELL CYCLE

Abstract
The seeding efficiency of colony-forming [mouse] cells from normal, regenerating and velocity-sedimented cycling and non-cycling marrow preparations was compared. Colony-forming cells in cycle exhibited a 50% reduction in splenic seeding when compared to normal marrow or sedimented non-cycling cells. The spleen colony assay apparently underestimates the total number of colony-forming cells by a fraction which is directly related to the number of cells in cycle.