β2‐Microglobulin Levels in Serum and Urine of Cadmium Exposed Rabbits

Abstract

Male rabbits were exposed to cadmium during 16 weeks by subcutaneous injections of either 0.25 mg or 0.5 mg Cd as cadmium chloride per kg body weight 3 times per week. β₂‐microglobulin (β₂‐m) and creatinine in serum, cadmium in blood, as well as total protein, creatinine, β₂‐m and cadmium in urine were determined before exposure and after 3 and 7 weeks of exposure. Measurements were also made at 19 weeks, 3 weeks after the last exposure. During exposure, there was a slight increase in the serum β₂‐m/creatinine ratio among rabbits with the highest exposure, while no effect of the cadmium burden could be observed once exposure had ceased. Urinary excretion of β₂‐m was related to urinary pH, which appeared to be the case also for excretion of total protein. In the high exposure group, a significant increase in urinary β₂‐m excretion, indicative of renal tubular dysfunction was seen after 7 weeks of exposure. This was, however, not related to serum β₂‐m levels. It was concluded that before renal damage has occurred even heavy cadmium exposure has very little influence on serum β₂‐m levels.