Maturation of glomerular size distribution profiles in domestic fowl (Gallus gallus)

Abstract

Previous histological evaluations of chick kidneys indicated nephrons continue to develop from embryonic foci for up to 6 weeks after hatching. The present study was conducted using an in vivo alcian blue staining technique to quantify posthatch changes in glomerular numbers and sizes in female domestic fowl at 1, 3, 5, 9, 12, 21, and 30 weeks of age. Changes in glomerular size distributions reflect changes in the heterogeneous nephron populations of avian kidneys. Foci of embryonic tissue were observed at the periphery of renal lobules up to 12 weeks of age. Glomerular numbers increased from 69,800/kidney at 1 week to 586,000/kidney at 12 weeks, with no further significant increase up to 30 weeks (599,000/kidney). The increase in glomerular number per gram kidney weight remained constant as kidney mass increased up to 12 weeks of age, after which the number of glomeruli per gram kidney weight declined significantly as kidney size increased without further addition of new nephrons. Glomerular size distribution profiles were constructed using eleven circumference categories. The peak number of glomeruli fell within the 0.11–0.14 mm category at 1 and 3 weeks; within the 0.15–0.18 mm category at 5, 9, and 12 weeks; and within the 0.19–0.22 mm category at 21 and 30 weeks. One and 3‐week‐old chicks had no glomeruli within the largest (⩾0.35 mm circumference) size categories, and 9–12‐week‐old birds had significantly fewer glomeruli in these categories than 21–30‐week‐old birds. These results demonstrate that posthatch renal maturation in domestic fowl involves the ongoing formation of new nephrons up to 12 weeks of age, with subsequent kidney growth (12–30 weeks of age) accomplished by enlargement of existing nephrons (nephron hypertrophy). The cumulative evidence indicates that nephrons destined to develop loops of Henle (mammalian‐type) develop first, with shorter (reptilian‐type) nephrons developing later as the kidneys enlarge.