Comparison of the Glycolytic Activity of Blood and Exudate Leucocytes

Abstract

The amount of lactic acid formed by blood and exudate leucocytes has been compared in the presence and absence of added glucose. The cells were collected from the same animals and in each instance the polymorphonuclear leucocyte was the predominant cell. It is shown that the metabolism of exudate leucocytes is altered so that they have a greater glycolytic activity than do blood leucocytes. The greater lactate formation by exudate leucocytes can be demonstrated routinely with glucose as substrate, and under particular experimental conditions can be shown to result from endogenous substrate in the absence of added glucose. Note: with the technical assistance of Anastasia Karistinos. Submitted on November 14, 1957