Rapid Extraction of DNA From Escherichia coli and Cryptosporidium parvum for Use in PCR
Open Access
- 1 November 2001
- journal article
- research article
- Published by American Society for Microbiology in Applied and Environmental Microbiology
- Vol. 67 (11), 5321-5324
- https://doi.org/10.1128/aem.67.11.5321-5324.2001
Abstract
The Xtra Amp tube, Isocode paper, Instagene matrix, and PrepMan matrix methods were evaluated for their ability to rapidly extract PCR-quality DNAs from Escherichia coli O157:H7 and Cryptosporidium parvum . All methods provided satisfactory DNA from E. coli , and the Xtra Amp and Instagene reagents provided satisfactory DNA from C. parvum .Keywords
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