Expression of human leukocyte antigen‐G and its correlation with interleukin‐10 expression in cervical carcinoma

Abstract

Objective: To determine human leukocyte antigen (HLA)‐G and interleukin (IL)‐10 expression and the relationship between HLA‐G expression and clinicopathologic features in patients with cervical cancer. Method: Tissue samples were obtained from 40 patients with cervical cancer and 15 control patients with a normal cervix. Quantitative real‐time RT‐PCR for mRNA and western blot analysis for protein expression were used. Result: Both HLA‐G and IL‐10 mRNA expression in cervical cancer tissues was significantly greater than normal controls (P < 0.001, P = 0.005). Protein expression of HLA‐G and IL‐10 in the cancer group was also significantly greater than in the controls (P < 0.001, P = 0.021). There was an inverse relationship between FIGO stage and HLA‐G mRNA expression (P = 0.046). Conclusion: HLA‐G and IL‐10 might play an important role in cancer progression of the cervix. High HLA‐G mRNA expression may be related to early carcinogenesis since it was associated with early‐stage cervical cancer.