An investigation into the solution structure of the single-stranded DNA undecamer 5?d AAGTGTGATAT by means of nuclear Overhauser enhancement measurements
- 1 October 1984
- journal article
- research article
- Published by Springer Nature in European Biophysics Journal
- Vol. 11 (2), 95-102
- https://doi.org/10.1007/bf00276624
Abstract
A 500-MHz 1H-NMR study on the single-stranded DNA undecamer (11-mer) 5′d AAGTGTGATAT is presented. Using a combination of one-dimensional pre-steady-state nuclear Overhauser enhancement (NOE) measurements and two-dimensional homonuclear J-correlated spectroscopy, virtually complete resonance assignments are obtained. The relative magnitudes of the intra- and internucleotide NOEs indicate that the overall structure of the single-stranded 11-mer is a right-handed B-type helix with extensive base stacking. Within this overall structure there is quite a large degree of variability, as exemplified by variations in glycosidic bond and sugar pucker conformations, most likely determined by base sequence.This publication has 41 references indexed in Scilit:
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