Segregation of Bad from Lipid Rafts Is Implicated in the Induction of Apoptosis
Open Access
- 1 April 2002
- journal article
- Published by The American Association of Immunologists in The Journal of Immunology
- Vol. 168 (7), 3387-3393
- https://doi.org/10.4049/jimmunol.168.7.3387
Abstract
Many molecules relocate subcellularly in cells undergoing apoptosis. Using coimmunoprecipitation experiments we demonstrate that Bad is not associated to 14-3-3 protein, suggesting a new mechanism for the control of the proapoptotic role of Bad. Here we show, by confocal microscopy and cellular fractionation, that Bad is attached to lipid rafts in IL-4-stimulated cells and thymocytes while associated with mitochondria in IL-4-deprived cells. Disruption of lipid rafts by methyl-β-cyclodextrin treatment induces segregation of Bad from rafts, which correlates with apoptosis. Our results suggest that the interaction of Bad with rafts is a dynamic process regulated by IL-4 and involved in the control of apoptosis.Keywords
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