Effects of Corticosteroid and 1,24R-Dihydroxy-Vitamin D3 Administration on Lymphoproliferation and Autoimmune Disease in MRL/MP-lpr/lpr Mice

Abstract

The pharmacological effects of prolonged administration of a corticosteroid, betamethasone, and active vitamin D₃ [1,24R–(OH)₂D₃] on lymphoproliferation and autoimmune disease of MRL/MP-lpr/lpr (MRL/l) mice were examined. Relatively high doses of betamethasone (0.25 mg/kg/day) prevented lymphoproliferation, reduced serum levels of anti-dsDNA, anti-ssDNA, and anti-poly (ADP-ribose) antibodies, and brought about clinical improvement, such as reduced proteinuria and diminution of skin lesions. It is noteworthy that not only did prevention of lymphoproliferation occur, but also recovery of the Lyt-2⁺ T cell subset in the thymus and the spleen was observed. The administration of 1,24R–(OH)₂D₃ (0.1 μg/kg/day) similarly prevented proteinuria, and produced recovery of a Lyt-2⁺ subset in the thymus.