Emergence of Competence (for Transformation) of Three Hemophilus Species in a Chemically Defined Environment.
- 1 December 1962
- journal article
- research article
- Published by Frontiers Media SA in Experimental Biology and Medicine
- Vol. 111 (3), 725-731
- https://doi.org/10.3181/00379727-111-27904
Abstract
Summary Three species of Hemophilus, grown under the method of Goodgal and Herriott, were shown to emerge to a state of competence for transformation to SM-resistance in a chemically defined medium: L-aspartic, L-glutamic acids in buffered saline containing calcium and magnesium ions. The degree of competence of a “standard'culture was enhanced many-fold in 30 to 60 minutes even when diluted 105-fold in the defined medium. The emergence of competence in the synthetic environment was temperature dependent and inhibited by chloramphenicol; multiplication was not necessary. Competent populations washed in the defined medium retain competence; in populations which have lost transformability as a result of washing in buffered saline, competence may be restored. Data presented suggest that the competence which emerges reflects the final attainment of the competent state rather than its “restoration'in cells which were competent at an earlier period during growth of the population.Keywords
This publication has 3 references indexed in Scilit:
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- Interspecific Transformation in Hemophilus; A Possible Index of Relationship Between H. Influenzae and H. aegyptius.Experimental Biology and Medicine, 1959
- THE NUTRITIONAL REQUIREMENTS OF HEMOPHILUS PARAINFLUENZAE 7901Journal of Bacteriology, 1949