Kinetics of Lead Citrate Staining of Thin Sections for Electron Microscopy
- 1 January 1983
- journal article
- research article
- Published by Taylor & Francis in Stain Technology
- Vol. 58 (1), 29-40
- https://doi.org/10.3109/10520298309066746
Abstract
The staining of thin sections [Psammechinus-miliaris testes, chicken erythrocyte and mouse liver] with lead citrate shows an initial increase followed by a decrease much later; the rate of the initial increase and subsequent loss varies for different cellular components. The decrease eventually reaches a stable minimum. At this level electron scattering is less than that of unstained sections, demonstrating a loss of biological material. Lead citrate used as a poststain following uranyl acetate causes an increase in electron density that is independent of staining time over 1-30 min; this increase appears to depend only on the quantity of uranyl acetate already bound, implying that the lead binds predominantly to the uranyl acetate.This publication has 9 references indexed in Scilit:
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