Microdetermination of Steroid Estrogens in Urine by Fluorometry.

Abstract

1. A quantitative method for the determination of estradiol, estrone and estriol in urine, based on the fluorescence of these substances when treated with phosphoric acid, is described. 2. The method is more accurate, sensitive and specific than other known biological or colorimetric tests. 3. The recoveries of estradiol were above 80%; of estrone between 70 and 80%; and of estriol between 50 and 60%. The mean experimental error was ± 10%. 4. The described method permits the determination of estriol in urine in quantities not hitherto detectable.