A study of the organization of apical dendrites in the somatic sensory cortex of the rat

Abstract

In the parietal cortex of the rat, sections cut tangentially show that profiles of medium and large apical dendrites are grouped into clusters. The number of apical dendrites in each cluster is variable and the usual separation between individual clusters is about 50 μ. Despite these variations the pattern does not appear to be random. Reconstructions from one micron serial sections show that neurons giving rise to the ascending dendrites forming clusters are located at different levels in layer V. The cell bodies of these neurons are arranged vertically below their dendrites and show a tendency to form groups. All of the neurons have apical dendrites that ascend through the cortex with a few secondary branches occurring close to the base. The principal secondary branching begins in layer III and spreads obliquely up through layer I. Furthermore, beginning in the inferior region of layer III apical dendrites are added to the clusters at their peripheries. These are from layer III pyramids. It is clear that the superior aspects of the cluster arrangements must intermingle with those of the neurons in adjacent clusters. The neuropil surrounding the dendrites forming clusters appears to contain a few smaller dendrites. Small unmyelinated axons are the most frequent component of the surrounding neuropil and these form terminals which synapse on the spines and trunks of the clustered dendrites. There is no obvious function that can be ascribed to the clusters other than they may form a component of the columnar organization in cortex described in part by physiological techniques.