An Automated p-Nitrophenylphosphate Serum Alkaline Phosphatase Procedure for the AutoAnalyzer

Abstract

A new and simplified procedure for the automated determination of serum alkaline phosphatase uses the AutoAnalyzer. The substrate p-nitrophenylphosphate in 2-amino-2-methyl-1-propanol offers the prime advantage of providing directly its own chromogen, p-nitrophenol, following enzyme action. The procedure also permits use of small sample volumes, provides linear reaction rates, and has a simple manifold design. Correlations are presented between the manual procedure and the automated system.