Extraction and identification of trophoblast cells circulating in peripheral blood during pregnancy

Abstract

Trophoblast cells circulating in peripheral venous blood during pregnancy were extracted by differential centrifugation and identified by indirect immunofluorescence with antitrophoblast microvillus antibody. When trophoblast identification was checked by blind comparison with non-pregnant control subjects, trophoblast cells were detected in 6 of the 10 pregnant women studied.