Correlation of retinal function with retinal histopathology following ischemia-reperfusion in rat eyes
- 1 January 2004
- journal article
- research article
- Published by Taylor & Francis in Current Eye Research
- Vol. 28 (6), 381-389
- https://doi.org/10.1080/02713680490503679
Abstract
Purpose. To investigate the relationship between retinal function and histopathology following retinal ischemia-reperfusion in rats. Methods. Retinal ischemia was induced in 27 Sprague-Dawley rats by raising the intraocular pressure for 60- or 90-minutes. Two weeks after the retinal ischemia-reperfusion, ganzfeld stimuli of different luminances (maximum 0.51 log cd-s/m2) were used to elicit full-field ERGs from the right (experimental) and left (control) eyes. The amplitudes of the scotopic b-waves were measured, and intensity-response curves were plotted. The b-wave amplitudes at the initial peak (VbIP), the dip (Vbdip), and the maximum stimulus intensity (Vbmax) were measured. The animals were sacrificed after the ERG recordings to determine the surviving retinal cells in the inner nuclear layer (INL). Results. The log values of the VbIP, Vbdip and Vbmax were linearly correlated with the number of surviving cells in the INL (r = 0.928, 0.905, and 0.913 for VbIP, Vbdip and Vbmax, respectively; p < 0.0001 for each). The slope of the regression line for the Vbdip was significantly steeper than that for the VbIP and Vbmax (p < 0.005). The regression line for the Vbdip reached undetectable level of the b-wave (Conclusions. The amplitude of the scotopic b-wave is a sensitive index of the surviving INL cells. However, if one selects the stimulus intensity at the dip, the functional loss would be exaggerated in spite of the presence of cells in the INL.Keywords
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