Regional glucose utilization and blood flow in experimental brain tumors studied by double tracer autoradiography
- 1 January 1985
- journal article
- research article
- Published by Springer Nature in Journal of Neuro-Oncology
- Vol. 3 (3), 271-283
- https://doi.org/10.1007/bf00165189
Abstract
Coupling of regional glucose utilizatio (GLU) and blood flow (CBF) was examined in rats with implanted brain tumors (AA ascites tumor) by quantitative double tracer autoradiography using 18F-2-fluorodeoxyglucose and 14C-iodoantipyrine. Four to 13 days after implantation, the animals were injected with the two tracers to obtain autoradiograms from the same brain section before and after the decay of 18F. The autoradiograms were then analyzed by an image processor to obtain a metabolic coupling index (MCI = GLU/ CBF). In the tumor, high GLU and low CBF were uncoupled to give a high MCI which implied anerobic glycolysis. In large tumors, the CBF was even lower. In the peri-tumoral region, GLU was reduced (especially in gray matter) and reduction was lowest around the larger tumors. CBF in the peri-tumoral region was also reduced, but this reduction became less as the distance from the tumor margin increased. These changes in the peri-tumoral region may be secondary to edema. The GLU and CBF of white matter was little influenced by the presence of tumors except for some reduction in these values in relation to the larger tumors. On a narrow margin of tumor and brain, corresponding to a zone of increased vascularity, CBF was moderately high. The MCI in the tumor was higher than in the cortex of the same as well as the opposite hemisphere. These findings indicate that the metabolism and blood flow of the tumor and surrounding brain are variable and directly related to tumor size.Keywords
This publication has 40 references indexed in Scilit:
- Use of short-lived 18F and long-lived 14C in double tracer autoradiography for simultaneous measurement of LCBF and LCGU.Stroke, 1984
- Autoradiographic determination of brain pH following middle cerebral artery occlusion in the rat.Stroke, 1984
- In vivo disturbance of the oxidative metabolism of glucose in human cerebral gliomasAnnals of Neurology, 1983
- New high-yield synthesis of 18F-labelled 2-deoxy-2-fluoro-D-glucoseThe International Journal of Applied Radiation and Isotopes, 1983
- Local Cerebral Blood Flow and Glucose Consumption of Rats with Experimental GliomasJournal of Cerebral Blood Flow & Metabolism, 1982
- Application of Quantitative Autoradiographic Measurements in Experimental Brain Tumor ModelsSeminars in Neurology, 1981
- Simultaneous measurement of blood flow and glucose metabolism by autoradiographic techniques.Stroke, 1981
- Use of 123I and 14C in a double radionuclide autoradiographic technique for simultaneous measurement of LCBF and LCMRgl. Theory and method.Stroke, 1981
- Metabolism of oxygen, glucose, lactate and pyruvate in the rat brain in situJournal of Neurochemistry, 1974
- ISOLATION OF A TUMOR FACTOR RESPONSIBLE FOR ANGIOGENESISThe Journal of Experimental Medicine, 1971