Fabrication of Ultrathin, Protein-containing Films by Layer-by-Layer Assembly and Electrochemical Characterization of Hemoglobin Entrapped in the Film
- 25 February 2003
- journal article
- Published by Oxford University Press (OUP) in Chemistry Letters
- Vol. 32 (3), 296-297
- https://doi.org/10.1246/cl.2003.296
Abstract
Multilayers of hemoglobin were uniformly deposited on a pyrolytic graphite electrode surface through a layer-by-layer self-assembly approach. The well-known anionic polyelectrolyte, DNA, was used to attract cationic hemoglobin due to the strong electrostatic interaction between DNA and the protein. Interestingly, the electron transfer reactivity of hemoglobin was significantly improved in the film, which in turn facilitated the electrocatalytic reduction of nitric oxide.This publication has 9 references indexed in Scilit:
- Hemin-Stimulated Docking of Cytochrome c to a Hemin−DNA Aptamer ComplexBiochemistry, 2002
- Nucleic Acid-Promoted Electron Transfer to Cytochrome cElectroanalysis, 2001
- Electron-Transfer Reactivity and Enzymatic Activity of Hemoglobin in a SP Sephadex MembraneAnalytical Chemistry, 2001
- The Peroxidase Activity of a Hemin−DNA Oligonucleotide Complex: Free Radical Damage to Specific Guanine Bases of the DNAJournal of the American Chemical Society, 2001
- Direct electrochemical characterization of the interaction between haemoglobin and nitric oxidePhysical Chemistry Chemical Physics, 2000
- Spectroelectrochemical investigation of direct electron transfer between resting horseradish peroxidase and its oxidation states promoted by DNAAnalytical and Bioanalytical Chemistry, 2000
- Fuzzy Nanoassemblies: Toward Layered Polymeric MulticompositesScience, 1997
- Electron Transfer between Electrodes and Heme Proteins in Protein−DNA FilmsJournal of the American Chemical Society, 1996
- Electrocatalytic reduction of nitrite and nitrosyl by iron(III) protoporphyrin IX dimethyl ester immobilized in an electropolymerized filmInorganic Chemistry, 1992