Algal Glyceraldehyde‐3‐Phosphate Dehydrogenase
Open Access
- 1 March 1976
- journal article
- Published by Wiley in European Journal of Biochemistry
- Vol. 63 (1), 155-161
- https://doi.org/10.1111/j.1432-1033.1976.tb10218.x
Abstract
Two enzymes with glyceraldehyde-3-phosphate dehydrogenase activity have been purified from heterotrophically grown Scenedesmus obliquus by ion-exchange chromatography and gel filtration. The D-enzyme has a molecular weight of 550000 and a VNADH: VNADPH ratio of 16 whereas the T-enzyme has a molecular weight of 140000 and a VNADH: VNADPH ratio of 0.15. The two enzymes, however, are very similar with regard to their Michaelis constants for the reduced pyridine nucleotides, pH optimum, subunit size and ultraviolet absorption.Keywords
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