Comparison of bromodeoxyuridine labeling indices obtained from tissue sections and flow cytometry of brain tumors
- 1 March 1988
- journal article
- research article
- Published by Journal of Neurosurgery Publishing Group (JNSPG) in Journal of Neurosurgery
- Vol. 68 (3), 388-392
- https://doi.org/10.3171/jns.1988.68.3.0388
Abstract
Sixteen patients with brain tumors were given a 30- to 60-minute intravenous infusion of bromodeoxyuridine (BUdR), 200 mg/sq m. Grossly viable fragments were taken from the biopsied tumor specimens and divided into two portions. One portion was dissociated into single cells, stained both with fluorescein isothiocyanate (FITC) using anti-BUdR monoclonal antibody as the first antibody and with propidium iodide (for deoxyribonucleic acid), and analyzed by flow cytometry (FCM). The labeling index (LI) was calculated as the number of FITC-labeled cells expressed as a percentage of the total number of cells analyzed. The other portion was fixed in 70% ethanol, embedded in paraffin, sectioned, and stained with immunoperoxidase using anti-BUdR monoclonal antibody as the first antibody. The LI of these tissue sections was calculated in two ways: from selected areas in which the labeled cells were evenly distributed and from the entire tissue section. The LI's obtained by FCM correlated closely with those from the ...Keywords
This publication has 29 references indexed in Scilit:
- Pediatric Central Nervous System Tumors: A Cell Kinetic Study with BromodeoxyuridineNeurosurgery, 1986
- Improved high-affinity monoclonal antibody to lododeoxyuridineCytometry, 1986
- The proliferative potential of human pituitary tumors in situJournal of Neurosurgery, 1986
- In situ cell kinetics studies on human neuroectodermal tumors with bromodeoxyuridine labelingJournal of Neurosurgery, 1986
- Monoclonal Antibody to 5-Bromo- and 5-Iododeoxyuridine: A New Reagent for Detection of DNA ReplicationScience, 1982
- The induction of specific-locus mutations and sister-chromatid exchanges by 5-bromo- and 5-chloro-deoxyuridineMutation Research, 1982
- Mutagenic mechanism of 5-bromodeoxyuridine in Chinese hamster cellsMutation Research, 1976
- Radiosensitization of Malignant Brain Tumours with Bromouridine (Thymidine Analogue)Acta Radiologica: Therapy, Physics, Biology, 1969
- Radiosensitization of Brain Tumor Cells with a Thymidine Analogue (Bromouridine)Journal of Neurosurgery, 1968