Determination of Bile Acids In Pharmaceutical Dosage Forms By Hplc
- 1 April 1989
- journal article
- pharmaceutical analysis
- Published by Taylor & Francis in Analytical Letters
- Vol. 22 (4), 915-927
- https://doi.org/10.1080/00032718908051377
Abstract
A rapid reversed-phase high-performance liquid chromatographic assay was developed for chenodeoxycholic acid and ursodeoxycholic acid in commercially available capsule and tablet formulations. A Supelcosil LC-18-DB column with UV detection at 210 nm and a methanol-acetate buffer eluent were used. Individual dosage forms were disintegrated in the mobile phase and an aliquot of the resulting suspension was filtered for the HPLC analysis. Recovery of the drugs from spiked placebo preparations was quantitative. the method was tested for linearity and specificity and was found to be simple, accurate and reproducible.Keywords
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