Labeling and Isolation of Plasma Membranes from Corn Leaf Protoplasts
- 1 June 1980
- journal article
- Published by Oxford University Press (OUP) in Plant Physiology
- Vol. 65 (6), 1053-1057
- https://doi.org/10.1104/pp.65.6.1053
Abstract
A plasma membrane-enriched fraction has been isolated from corn leaf mesophyll protoplasts and its identity confirmed with the aid of an external label, diazotized [125I]iodosulfanilic acid. Gentle cell disruption enabled internal organelles to be maintained intact and thus facilitated separation from the plasma membrane. The plasma membrane-enriched fraction was devoid of chloroplast or mitochondrial markers, whereas markers for the endoplasmic reticulum and golgi indicated minimal contamination. The highly enriched plasma membrane fraction contained a Mg2+-dependent, K+-stimulated ATPase with a pH optimum near neutrality. The position of the membranes on sucrose density gradients indicates that the plasma membranes have characteristics similar to other plasma membrane fractions.Keywords
This publication has 15 references indexed in Scilit:
- Diazotized [125I]diiodosulfanilic acid as a label for cell surface membranes. Studies on erythrocytesBiochimica et Biophysica Acta (BBA) - Biomembranes, 1979
- A modification of the Lowry procedure to simplify protein determination in membrane and lipoprotein samplesAnalytical Biochemistry, 1978
- The isolation of plasma membrane from protoplasts of soybean suspension culturesJournal of Cell Science, 1977
- β-Glucan Synthetases of Plasma Membrane and Golgi Apparatus from Onion StemPlant Physiology, 1974
- Evidence for the Presence of the Toxin-Binding Protein on the Plasma Membrane of Sugarcane CellsProceedings of the National Academy of Sciences, 1974
- [36] Purification of a plasma membrane-bound adenosine triphosphatase from plant rootsMethods in Enzymology, 1974
- Purification of Enzymatically Isolated Mesophyll Protoplasts from C3, C4, and Crassulacean Acid Metabolism Plants Using an Aqueous Dextran-Polyethylene Glycol Two-Phase SystemPlant Physiology, 1973
- Purification of an Ion-Stimulated Adenosine Triphosphatase from Plant Roots: Association with Plasma MembranesProceedings of the National Academy of Sciences, 1972
- ISOLATION OF β-GLUCAN SYNTHETASE PARTICLES FROM PLANT CELLS AND IDENTIFICATION WITH GOLGI MEMBRANESProceedings of the National Academy of Sciences, 1969
- A fluorescent label for the outer components of the plasma membraneBiochimica et Biophysica Acta (BBA) - Specialized Section on Biophysical Subjects, 1964