3'-terminal nucleotide sequence of encephalomyocarditis virus RNA determined by reverse transcriptase and chain-terminating inhibitors.
- 1 September 1978
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 75 (9), 4257-4261
- https://doi.org/10.1073/pnas.75.9.4257
Abstract
The chain-termination method for determining the nucleotide sequence of DNA of Sanger, Nicklen and Coulson was adapted for use with reverse transcriptase (RNA-directed DNA nucleotidyltransferase) on RNA templates. With this method and using a primer (the octanucleotide pdT7rC) directed at the 3''-terminal poly(A), a sequence of 166 residues was determined in the genomic RNA of the picornavirus encephalomyocarditis virus.This publication has 17 references indexed in Scilit:
- The 3′‐Terminal Nucleotide Sequence of Encephalomyocarditis Virus RNAEuropean Journal of Biochemistry, 1978
- New rapid gel sequencing method for RNANature, 1977
- Nucleotide sequence of part of the gene for human chorionic somatomammotropin: Purification of DNA complementary to predominant mRNA speciesCell, 1977
- Rapid gel sequencing of RNA by primed synthesis with reverse transcriptaseJournal of Molecular Biology, 1977
- The primary structure of rabbit β-globin mRNA as determined from cloned DNACell, 1977
- Sequence analysis of the 3′ non-coding regions of rabbit α- and β-globin messenger RNAsJournal of Molecular Biology, 1976
- A rapid method for determining sequences in DNA by primed synthesis with DNA polymeraseJournal of Molecular Biology, 1975
- Tumor virus ribonucleic acid directed deoxyribonucleic acid synthesis. Nucleotide sequence at the 5' terminus of nascent deoxyribonucleic acidBiochemistry, 1974
- Infection of mouse fibroblasts by cardioviruses: Premature uncoating and its prevention by elevated pH and magnesium chlorideVirology, 1971
- Ribonucleic Acid Content of Encephalomyocarditis VirusJournal of General Virology, 1970