Complete Development of Mosquito Phases of the Malaria Parasite in Vitro

Abstract

Methods for reproducible in vitro development of the mosquito stages of malaria parasites to produce infective sporozoites have been elusive for over 40 years. We have cultured gametocytes ofPlasmodium bergheithrough to infectious sporozoites with efficiencies similar to those recorded in vivo and without the need for salivary gland invasion. Oocysts developed extracellularly in a system whose essential elements include co-culturedDrosophilaS2 cells, basement membrane matrix, and insect tissue culture medium. Sporozoite production required the presence of para-aminobenzoic acid. The entire life cycle ofP. berghei, a useful model malaria parasite, can now be achieved in vitro.