INTRACELLULAR HYDRION CONCENTRATION STUDIES

Abstract

Injection of a graded series of phosphate buffers into amebae previously injected with hydrion indicators shows that the cytoplasm has considerbale buffering power. The intraprotoplasmic pH is constant and can not be changed so long as the cell is still living. A method for checking the pH values obtained by direct color comparisons depends upon the injection of colorless buffer solutions into amebae colored with indicators and observing the temporary changes in color produced. Results harmonize with those of investigators who place the Ph of Ameba at 6.9.