DNA polymerase activities in growing cells infected with simian virus 40

Abstract

Growing [African green monkey kidney] CV1 cells were infected with SV-40, and the levels of DNA polymerases-.alpha., -.beta., and -.gamma. were analyzed in the cytoplasm, nuclear Triton wash and nucleus. In the cytoplasmic fraction, the amount of .alpha.-, .beta.-, or .gamma.-polymerase remained unaltered after SV-40 infection. The activity of DNA polymerase-.alpha. increased 5- to 6-fold in the nuclear Triton wash and 3-fold in the nuclei and then remained enhanced only inside the nuclei. That of DNA polymerase-.beta. and -.gamma. increased mostly in the nuclei after infection. DNA polymerase-.alpha. could be the major enzyme involved in SV-40 DNA replication.