Studies on peptides. LIX. Synthesis of the nonatriacontapeptide corresponding to the entire amino acid sequence of porcine adrenocorticotropic hormone.

Abstract
The nonatriacontapeptide corresponding to the newly revised amino acid sequence of porcine ACTH was synthesized by successive condensations of 4 peptide fragments; Z(OCH3)-(15-19)-OH, Z(OCH3)-(11-14)-OH, Z(OCH3)-(5-10)-OH and Z-(1-4)-NHNH2, with H-(20-39)-OC7H7, a synthetic intermediate of porcine corticotropin-like intermediate lobe peptide. The synthetic peptide exhibited the identical Rf value with that of natural porcine ACTH in 2 different solvent systems and its in vivo steroidogentic activity was 148.2 IU/mg.

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