GROWTH CONTROL OF DIFFERENTIATED FETAL RAT HEPATOCYTES IN PRIMARY MONOLAYER CULTURE
Open Access
- 1 September 1974
- journal article
- Published by Rockefeller University Press in The Journal of cell biology
- Vol. 62 (3), 767-779
- https://doi.org/10.1083/jcb.62.3.767
Abstract
Dialyzed fetal bovine serum contains two distinct growth-controlling macromolecular fractions: one stimulates and the other inhibits proliferation of primary cultured differentiated fetal rat hepatocytes. Both fractions are precipitated by ammonium sulfate (50% saturation, pH 7.4, 4 degrees C). Serum fraction I (SFI, mol wt >/= 120,000 daltons estimated by gel filtration with Bio-gel P200) appears to contain at least two factors which function, respectively, to initiate DNA synthesis (activity pH 4-10 stable) and to increase the rate at which initiated cells traverse the cell cycle (activity pH 4 and pH 10 labile). Intraperitoneal injections of SFI into adult rats have produced detectable stimulation of hepatic but not renal DNA synthesis. Serum fraction II (SFII, mol wt 40,000-80,000 daltons) suppresses in vitro incorporation of CH(3)-[(3)H]thymidine into DNA under conditions which diminish neither cell viability nor cell attachment. Mixing experiments indicate that SFI and SFII mutually antagonize each other with respect to DNA synthesis and cell multiplication. Thus, both the relative and absolute serum levels of multiple factors control in vitro fetal hepatocyte proliferation.Keywords
This publication has 26 references indexed in Scilit:
- GROWTH CONTROL OF DIFFERENTIATED FETAL RAT HEPATOCYTES IN PRIMARY MONOLAYER CULTUREThe Journal of cell biology, 1974
- Serum dependent growth of primary cultured differentiated fetal rat hepatocytes in arginine-deficient mediumJournal of Cellular Physiology, 1973
- STUDIES ON PRIMARY CULTURES OF DIFFERENTIATED FETAL LIVER CELLSThe Journal of cell biology, 1972
- Stimulation of DNA and Protein Synthesis in Fetal-Rat Liver Cells by Serum from Partially Hepatectomized RatsProceedings of the National Academy of Sciences, 1972
- Regeneration of Rat Liver: Transfer of Humoral Agent by Cross CirculationScience, 1967
- The initiation of cell division in a contact‐inhibited mammalian cell lineJournal of Cellular and Comparative Physiology, 1965
- TISSUE CULTURE POPULATIONS AND THEIR RELATION TO THE TISSUE OF ORIGINProceedings of the National Academy of Sciences, 1960
- Growth Factors for Mammalian Cells in CultureJournal of Biological Chemistry, 1959
- Nutrition Needs of Mammalian Cells in Tissue CultureScience, 1955
- FETUIN: THE MUCOPROTEIN OF FETAL CALF SERUMJournal of Biological Chemistry, 1954