PhiC31 integrase induces efficient site-specific excision in zebrafish
- 17 June 2010
- journal article
- research article
- Published by Springer Nature in Transgenic Research
- Vol. 20 (1), 183-189
- https://doi.org/10.1007/s11248-010-9394-5
Abstract
Site-specific recombinases catalyze recombination between specific targeting sites to delete, insert, invert, or exchange DNA with high fidelity. In addition to the widely used Cre and Flp recombinases, the phiC31 integrase system from Streptomyces phage may also be used for these genetic manipulations in eukaryotic cells. Unlike Cre and Flp, phiC31 recognizes two heterotypic sites, attB and attP, for recombination. While the phiC31 system has been recently applied in mouse and human cell lines and in Drosophila, it has not been demonstrated whether it can also catalyze efficient DNA recombination in zebrafish. Here we show that phiC31 integrase efficiently induces site-specific deletion of episomal targets as well as chromosomal targets in zebrafish embryos. Thus, the phiC31 system can be used in zebrafish for genetic manipulations, expanding the repertoire of available tools for genetic manipulation in this vertebrate model.Keywords
This publication has 19 references indexed in Scilit:
- FlEx‐based transgenic reporter lines for visualization of Cre and Flp activity in live zebrafishGenesis, 2009
- Mutational Derivatives of PhiC31 Integrase With Increased Efficiency and SpecificityMolecular Therapy, 2009
- Recombinases and Their Use in Gene Activation, Gene Inactivation, and TransgenesisPublished by Springer Nature ,2008
- Gene trap and gene inversion methods for conditional gene inactivation in the mouseNucleic Acids Research, 2005
- A Transposon-Mediated Gene Trap Approach Identifies Developmentally Regulated Genes in ZebrafishDevelopmental Cell, 2004
- Construction of Transgenic Drosophila by Using the Site-Specific Integrase From Phage C31Genetics, 2004
- Transgene Manipulation in Zebrafish by Using RecombinasesMethods in Cell Biology, 2004
- Talking about a RevolutionDevelopmental Cell, 2004
- Phage Integrases: Biology and ApplicationsJournal of Molecular Biology, 2003
- Site-specific cassette exchange and germline transmission with mouse ES cells expressing φC31 integraseNature Biotechnology, 2003