Kinetics of multilayer immobilized enzyme‐filter reactors: Behavior of urease‐filter reactors in different buffers

Abstract
A sequential model which describes the kinetic behavior of multilayer immobilized-enzyme filter reactors for systems obeying the Michaelis-Menten scheme and various types of inhibition by product and by substrate is proposed. The model was varified experimentaly using a single enzyme-filter-substrate system—urease bound to nylon-filter disks acting on urea—in different buffers to obtain the various inhibition patterns.