A NEW TECHNIQUE FOR THE SIMULTANEOUS DEMONSTRATION OF LIPID AND ACID POLYSACCHARIDES ON THE SAME TISSUE SECTION
Open Access
- 1 March 1959
- journal article
- research article
- Published by SAGE Publications in Journal of Histochemistry & Cytochemistry
- Vol. 7 (2), 101-106
- https://doi.org/10.1177/7.2.101
Abstract
A new combination staining technique which utilizes alcian blue for acid polysaccharides and oil red O in carbowax "400" for lipid is described. This technique, combined with a carbowax embedding procedre, provides a method which fulfills all of the requirements for the study of these substances in the same tissue section; i.e., (1) lipid and polysaccharide loss during the preparation of the tissue for staining is minimal, (2) the lipid stains sharply, (3) the polysaccharide stain is specific, (4) the stains contrast sharply, (5) the lipids and polysaccharides are identifiable "in statu nascendi", and (6) thin adjacent sections can routinely be prepared.Keywords
This publication has 9 references indexed in Scilit:
- THE SPECIFICITY OF THE PERIODIC ACID-SCHIFF TECHNIQUE STUDIED BY A QUANTITATIVE TEST-TUBE METHODJournal of Histochemistry & Cytochemistry, 1957
- APPLICATION OF ALCIAN BLUE AS A HISTOCHEMICAL METHOD1957
- Two Organic Fixatives for Acid MucopolysaccharidesStain Technology, 1956
- The Reactions of Isolated Mucopolysaccharides to Several Histochemical TestsStain Technology, 1955
- Histochemical analysis of mucopolysaccharides occurring in mucus-producing tumors.Mixed tumors of the parotid gland, colloid carcinomas of the breast, and myxomasCancer, 1952
- Specificity of Staining Methods for Mucopolysaccharides of the Hyaluronic Acid TypeStain Technology, 1952
- The chemical histology of the human placenta and decidua with reference to mucopolysaccharides, glycogen, lipids and acid phosphataseJournal of Anatomy, 1948
- THE BIOLOGICAL SIGNIFICANCE OF HYALURONIC ACID AND HYALURONIDASEPhysiological Reviews, 1947
- Histochemical Demonstration of Acid Polysaccharides in Animal TissuesNature, 1946