Effects of LH-RH agonist or LH-RH immunoneutralization on pituitary and ovarian LH-RH receptors in female rats

Abstract

Summary. Low levels of gossypol inhibited motility and anaerobic glycolysis of ejaculated rhesus monkey spermatozoa. Inhibition (50%) of both occurred at a gossypol:sperm ratio of 8 nmol/10⁸ spermatozoa, and complete inhibition of both occurred at a ratio of 75 nmol/10⁸ spermatozoa. Determination and comparison of the levels of glycolytic intermediates in intact spermatozoa, incubated with and without gossypol, indicated that the only site of glycolytic inhibition was lactate dehydrogenase-X (EC 1.1.1.27). At gossypol:sperm ratios above 6 nmol/10⁸ spermatozoa, gossypol also decreased the concentrations of the adenine nucleotides, ATP, ADP and AMP, and this is most probably the basis for its toxic effect on spermatozoa.