Immunosuppression in Man: Suppression by Macrophages Can be Mediated by Interactions with Regulatory T Cells
- 1 September 1977
- journal article
- research article
- Published by The American Association of Immunologists in The Journal of Immunology
- Vol. 119 (3), 918-924
- https://doi.org/10.4049/jimmunol.119.3.918
Abstract
Previous data from this laboratory indicated that profound defects of in vivo and in vitro T cell function noted in some patients with fungal infection reflected the presence of immunosuppressive forces. Investigation of the cellular interactions required for this immunosuppression to become manifest revealed the following. Patients' macrophages liberated, in vitro, a soluble material (SM) 3 capable of inhibiting the blastogenic response of normal T cells to mitogens and antigens. However, not all T cells were equally susceptible to its suppressive effect. Incubation of normal PBMC in culture fluid for 7 days yielded a population of cultured T cells whose reactivity could not be inhibited by SM. Binding studies indicated that this “resistance” did not reflect any gross alterations in the ability of cultured T cells to interact with SM. Rather, it reflected the functional deletion of a subpopulation of T cells required for SM to mediate its suppressive effect. This was demonstrated by the finding that in the presence of low density, fresh, autologous T cells, SM was capable of suppressing the reactivity of cultured T cells. Similar interactions between SM and low density T cells appeared to be required for SM to inhibit T-dependent responses among fresh noncultured PBMC. Thus, removal of low density T from fresh normal PBMC yielded high density T whose reactivity was not suppressed by SM. These studies are interpreted as indicating that immunosuppression in the patients studied does not reflect direct interactions between suppressive macrophages and responding T cells. Instead, it represents an indirect interaction whereby macrophages may generate or potentiate active suppressive T cells.This publication has 6 references indexed in Scilit:
- Suppressor cell influence in selected strains of inbred ratsCellular Immunology, 1977
- Generation of T-helper cells in vitro. I. Cellular and antigen requirements.The Journal of Experimental Medicine, 1977
- FUNCTIONAL CHARACTERISTICS OF MONOCYTESTransplantation, 1977
- Nature of the antigenic complex recognized by T lymphocytes. I. Analysis with an in vitro primary response to soluble protein antigens.The Journal of Experimental Medicine, 1976
- Site of Action of a Soluble Immune Response Suppressor (SIRS) Produced by Concanavalin A-Activated Spleen CellsThe Journal of Immunology, 1976
- Mechanisms of genetic resistance to Friend virus leukemia in mice. II. Resistance of mitogen-responsive lymphocytes mediated by marrow-dependent cells.The Journal of Experimental Medicine, 1976