Identification of glycoproteins associated with elastin-associated microfibrils.
Open Access
- 1 April 1985
- journal article
- research article
- Published by SAGE Publications in Journal of Histochemistry & Cytochemistry
- Vol. 33 (4), 287-294
- https://doi.org/10.1177/33.4.3980982
Abstract
The microfibrils associated with elastic tissue have been shown to be predominantly proteinaceous. On the basis of their affinity for cationic stains, including ruthenium red, they have been assumed to be glycoprotein, but more evidence to support this claim has not been adduced. Despite repeated investigation of glycoprotein materials obtained by extraction of elastic tissues with reagents that appear to remove microfibrils, the chemical composition of elastin-associated microfibrils remains obscure. An electron microscopic study of the microfibrils in two elastin-rich tissues (bovine nuchal ligament and aorta) during their development was pursued using more specific histochemical methods. The periodic acid-alkaline bismuth stain (analogous to the periodic acid-Schiff stain for glycoproteins in light microscopy) has been adapted for this study. Specific aldehyde groups (confirmed by blocking with m-aminophenol or sodium borohydride) were identified after periodate oxidation as fine granules of bismuth stain. These were shown to localize specifically along the elastin-associated microfibrils in a finely punctate form. Staining of the amorphous elastic component did not occur except for a fine rim adjacent to the microfibrils. Lectin binding with concanavalin A (with ferritin markers) confirmed that there are glucose- or mannose-containing proteins associated with the microfibrillar component of elastic tissue. This was true of these microfibrils in all layers of the aortic wall and throughout the ligament. It was also true of mature adult tissues in which there was a lesser proportion of microfibrils. It is concluded that elastin-associated microfibrils really are associated with glycoprotein(s).This publication has 16 references indexed in Scilit:
- Histochemical and ultrastructural characterization of subendothelial glycoprotein microfibrils interacting with platelets.Journal of Histochemistry & Cytochemistry, 1982
- The nature of the microfibrillar glycoproteins of elastic fibres. A biosynthetic studyBiochemical Journal, 1981
- Elastin-associated microfibrils in aorta: species differences in large animalsMicron, 1981
- The identification of glycoproteins associated with elastic-tissue microfibrilsBiochemical Journal, 1978
- PREPARATION OF A SPECIFIC ANTISERUM TOWARDS MICROFIBRILLAR PROTEIN OF ELASTIC TISSUES1977
- CARDIOVASCULAR STUDIES ON COPPER-DEFICIENT SWINE .10. FINE STRUCTURE OF DEFECTIVE ELASTIC MEMBRANES1967
- Observations on the morphology of the proteinpolysaccharide complex of bovine nasal cartilage and its relationship to collagenProceedings of the Royal Society of London. B. Biological Sciences, 1966
- Ultrastructural studies on early elastogenesisThe Anatomical Record, 1966
- THE FINE STRUCTURE OF ELASTIC FIBERSThe Journal of cell biology, 1966
- A MICROCHEMICAL REACTION RESULTING IN THE STAINING OF POLYSACCHARIDE STRUCTURES IN FIXED TISSUE PREPARATIONS1948